How does the variations in protocols used to prepare various platelet concentrates affect the release of growth factors, cytokines and matrix proteins?
The population/problem for the proposed review would be the variations in protocols used to prepare various forms of platelet concentrates.
Platelet concentrates are known as blood derivatives rich in growth factors, cytokines and matrix proteins. The release of these bioactive mediators positively affect the wound healing process and bone regeneration. There are various forms of platelet concentrates based on the protocols used in preparation by varying the centrifugation speed(rcf/rpm), number of cycles, time, test tubes and additives used etc.
( The platelet concentrates included in this study are fibrin glue, fibrin sealant, platelet rich plasma(PRP), concentrated growth factor(CGF), plasma rich in growth fcators(PRGF), platelet rich fibrin(PRF), platelet rich fibrin matrix(PRFM), leuokocyte and platelet rich fibrin(L-PRF), advanced platelet rich fibrin(A-PRF), injectable platelet rich fibrin(i-PRF), titanium platelet rich fibrin(T-PRF).
Quantification and duration of release of growth factors, cytokines and matrix proteins.
The quantification of the factors through ELISA/PCR/Flow Cytometry along with the duration of release will be compared between various platelet concentrates and within different protocols.